免疫乳抗炎作用研究

ANTI-INFLAMMATORY EFFECTS OF IMMUNE MILK

目的:研究免疫乳的抗炎症作用。方法:以24株人肠道病原菌(包括病原性大肠杆菌12株、沙门菌8株、志贺菌3株、小肠结肠炎耶尔森菌1株)作为抗原,对乳牛进行系统免疫,免疫乳与非免疫乳乳中IgG含量无显著差异。结果:系统免疫并不增加乳中IgG的含量,但IgG的抗体特异性大大增强;免疫乳(immunemilk,IM)中乳抗体对24种不同病原菌的凝集价为128,为普通乳(regularmilk,RM)中乳抗体凝集价的64倍。结论:免疫乳对角叉菜胶和甲醛致炎引起的大鼠足趾肿胀具有显著的抑制作用,同时可显著降低炎性组织中PGE2的含量。免疫乳粉对小鼠肉芽肿也具有显著的抑制作用。

Objective： To study the anti-inflammatory effect of immune milk. Method： Twenty four strains of pathogenic bacteria （including 12 strains of pathogenic E.coli, 8 strains of Salmonella, 3 strains of Shigella and 1 strain of Yersinia enterocolitica） originated from human intestinal tract were selected as antigens, and dairy cows were immunized. Results： Vaccination could not increase the concentration of IgG in the immune milk, but significantly highten the specificity of milk antibodies. The antibody titers against 24 pathogenic bacteria in immune milk was 128, 64 folds of control regular milk. Conclusion： Feeding of immune milk could inhibit edema of rat footpad induced by carrageenin and formaldehyde and the formation of acestoma of mice caused by cotton embedded hypophloedally. Meanwhile, the PGE2 level in inflammatory footpad of rat fed with immune milk was remarkably lower than that of rat fed with regular milk. These results indicate that immune milk has anti-inflammatory property.