柱花草组织培养研究

On Tissue Culture of Stylosanthes

以6个柱花草品种为材料,研究了柱花草愈伤组织的诱导、芽和根的分化等组织培养技术,结果表明,外值体和培养基对柱花草组织培养的影响较大.愈伤组织的诱导,6个品种之间无显著性差异,但不同的外值体却表现出极显著的差异,其中真叶的出愈率最高;芽和根的分化,真叶的分化系数、生根率和生根系数最高,最佳芽和根的分化培养基分别为MS+1.0 mg/L NAA+4.0 mg/L KT,1/2MS+1.0 mg/L NAA+0.5 mg/L KT.

A study the tissue culture technique of the inducing callus, and buds and roots differentiation on six different Stylosanthes, showed that explants and medium have bigger to the tissue culture of Stylosanthes. In the inducing callus, they have not remarkable difference among the six varieties, but have the remarkable difference among the different explants and the inducing-callus rate of the first outspread foliage was the highest. In the bud,; and roots differentiation, the dedifferentiation coefficient, the rhizogenesis rate and the rhizogenesis coefficient of the first outspread foliage was the highest, and the best buds and roots differentiation medium was respectively MS＋1.0 mg/L NAA＋ 4.0 mg/L KT and 1/2MS＋1.0 mg/L NAA＋ 0.5 mg/L KT.