摘要
乙醛脱氢酶(ALDH)是一种胞内酶,在从酵母中分离提取ALDH的过程中,破碎细胞是其中的关键步骤之一。本文着重考察了自溶、反复冻融、超声波和高压破碎仪的细胞破碎方法对释放ALDH的影响。结果表明,用高压破碎仪破碎,酵母细胞破碎率可达到95%以上,而且有效地防止了ALDH的分解及降解,是一种较为理想的破碎方法。
Aldehyde dehydrogenase （ALDH） is a kind of endoenzyme and cell-breaking is one of the key procedures in the extraction of ALDH from S.cerevisiae. The effects of different cell-breaking methods including self-dissolving, repetitive freezing and thawing and ultrasonication and high pressure cracker on ALDH release were studied and compared in this paper. The results showed that cell-breaking by high press cracker was the best methord with its breaking rate above 95 % and it could effectively prevent ALDH decomposition and degradation.
出处
《酿酒科技》
北大核心
2006年第11期21-23,共3页
Liquor-Making Science & Technology
基金
湖北省新世纪高层次人才工程入选人员科研项目择优资助
新世纪百千万人才工程国家级人选科研项目资助
湖北省国际科技合作重点项目计划
关键词
微生物
酵母细胞
乙醛脱氢酶
破碎方法
microbe
yeast cells
aldehyde dehydrogenase
breaking methods
