离子注入与紫外诱变筛选产高级醇低的果酒酵母

Screening of Fruit Wine Yeast With Low Higher Alcohol Yield by Ion Implantation and UV Mutagenesis

采用离子注入法对一株自黎族酒曲中分离得的酵母TQ601诱变选育,通过乳酸培养基、产酸培养基和TTC上层平板初筛,再经果汁发酵复筛,并用气相色谱法测定果酒中高级醇含量,得到16株菌,其中TQ105菌的高级醇产量降低了34.5%。为防止发生回复突变,选择TQ111菌对其进行紫外诱变以巩固诱变效果。最终得到7株菌高级醇的产量均低于原始菌种TQ601,而且遗传稳定性较好。

By ion implantation of an original strain TQ601 （separated from starter of Ni Minority） and then petri-dish screening on lactic acid, YEPD-CaCO3 and TTC and then secondary screening through fruit juice fermentation, 16 strains with low higher alcohol yield were eventually obtained. The higher alcohol yield of all the 16 strains was measured by gas chromatography. Higher alcohol yield of TQ105 strain decreased by 34.5 %. To prevent resorting mutation, TQ111 strain was selected for UV mutagenesis and 7 strains with low higher alcohol yield and satisfactory heredity stability （their higher alcohol yield lower than original strain TQ601） were finally obtained.