摘要
目的研究膀胱癌中,RASSF2A、NORE1A基因启动子甲基化状态在肿瘤发生发展中的作用。方法采用甲基化特异性PCR(methylation specific PCR,MSP)技术检测54例膀胱癌组织及其相应癌周正常组织RASSF2A、NORE1A基因启动子甲基化状态。结果(1)RASSF2A检出率仅为2/54,NORE1A仅为0/54;(2)相应癌周正常组织和3例正常膀胱组织均未发现该基因高甲基化改变;(3)甲基化状态与膀胱癌临床病理参数无明显相关性。结论RASSF2A、NORE1A在膀胱肿瘤中的甲基化率较低,并不适合进行临床筛查。
Objective To investigate the methylation bladder carcinoma and its role in tumorigenesis. Methods status of the promoter region of RASSF2A and NORE1A in The methylation status of promoter regions of RASSF2A and NORE1A was examined by MSP in 54 bladder carcinoma tissues and adjacent normal tissues from 54 cases. Results ( 1 ) 2/54 of bladder carcinoma tissues showed RASSF2A hypermethylation, and NORE1A showed 0/54. (2) None of paired adjacent normal tissues and three normal bladder tissues showed RASSF2A and NORE1A hypermethylation. (3) There was no significant correlation between the methylation status with the clinical pathological parameters in bladder carcinoma. Conclusion Hypermethylation rates of RASSF2A and NORE1A in bladder carcinoma are low, which suggest it is unsuitable for clinical screening.
出处
《同济大学学报(医学版)》
CAS
2006年第5期34-36,共3页
Journal of Tongji University(Medical Science)