组织因子与产科凝血关系的初步研究

A primary study on the relationship between tissue factor and obstetrical coagulation

目的检测正常足月妊娠胎盘、蜕膜和子宫肌层中是否富含在启动凝血、止血过程中起关键作用的组织因子(tissuefactor,TF),初步探讨TF与产科凝血间的关系。方法选择正常足月妊娠30例,用ELISA方法分别检测血浆、胎盘、蜕膜、子宫肌层中TF抗原含量。结果胎盘、蜕膜和子宫肌层中TF抗原含量分别为(35.43±1.51)ng/ml(14.17±0.60)ng/mg总蛋白、(39.00±1.88)ng/ml、(15.60±0.75)ng/mg总蛋白和(10.92±3.19)ng/ml(4.37±1.28)ng/mg总蛋白。血浆中为0.013ng/mg总蛋白。胎盘、蜕膜、子宫肌层的TF抗原含量分别是血浆的1090、1200、336倍。结论正常足月妊娠胎盘、蜕膜和子宫肌层富含TF,TF可能与胎盘剥离面凝血止血及产科凝血障碍有关。

Objective To explore the relationship between obstetrical coagulation and tissue factor （TF）, the key component of the initiation of coagulation. Methods TF antigen was measured in serum, placenta, decidua and myometrium by immunoenzymatic method （ELISA） in 30 healthy pregnancy women. Results Concentrations of TF antigen in placenta, decidua and myometrium were （35.43±1.51） ng/ml （14. 17±0.60） ng/mg of total protein）, （39. 005=1.88） ng/ml （15.60±0. 75） ng/mg of total protein） and （10.92±3.19） ng/ml （4.37±1.28） ng/mg of total protein）, respectively. In serum, it was 0. 013 ng/mg of total protein. The levels of TF antigen in placenta, decidua and myometrium were 1 090 times, 1 200 times, 336 times higher than in blood. Conclusions Normal placenta, decidua and myometrium are significantly abundant of TF, which is closely related to coagulation. Therefore, obstetrical coagulation disturbances may be associated with abnormal TF at the surface of placenta separation.