HPLC法同时测定五维B颗粒中的烟酰胺、维生素B_1、维生素B_2的含量

Simultaneous determination of nicotinamide、vitamin B_1、vitamin B_2 in five vitamin granules by HPLC

目的:建立同时测定五维B颗粒中维生素B1、维生素B2、烟酰胺三组分含量的高效液相色谱法。方法:采用ODS C18柱、柱长:150×4.6mm流动相为甲醇:乙腈:0.06%己烷磺酸钠和0.04%庚烷磺酸钠的混合液:冰醋酸=45:30:24:1流速:1.2mL/min柱温3:0℃波长:275nm,外标法计算。结果:线性范围分别是:烟酰胺90￣210μg/mL;r=0.999维生素B2 12￣32μg/mL;r=0.999、维生素B1 25￣65μg/mL;r=0.998,回收率分别为:烟酰胺99.5%、维生素B2 99.7%、维生素B1 100.3%。结论:本方法分离效果好、辅料无干扰、快速、简便、准确适合于该制剂3组分的同时测定。

Objective to establish a method for Simultaneous determination of three constituents nicotinamide, vitamin B1,vitamin B2,in compound five vitamin granules. Methods HPLC analytical column was ODS C18 column, the mobile phase, CH3OH：CH3CN：a mixture of 0.06%C6H13 SO3 Na and 0.04%C7H15 SO3 Na：CHsCOOH=45 ：30：24：1,The flow rate was 1.2mL/min.the UV detection wavelength was 275nm Result the linear ranges of three constituents were nicotinamide 90-210μg/mL r=0.999 Vitamin B2 12-32μg/mL;r=0.999, Vitamin B1 25-65μg/mL;r=0.999, respectively. Conclusion The method is simple. This method is suitable for the quality control of the compound preparation.