BFF、rbGH对牛卵泡卵母细胞体外受精后发育的影响

Effects of BFF, rbGH on Development after the IVF of Follicular Oocytes in Bovine

利用屠宰黄牛卵巢，对２～５ｍｍ卵泡卵母细胞的体外成熟（ＩＶＭ）、体外受精（ＩＶＦ）、受精卵的体外培养（ＩＶＣ）进行了系列研究。结果表明，在成熟培养液中单独添加１０％Ｄ０ＥＣＳ（发情当天牛血清）获得了卵泡卵母细胞受精后较高的卵裂率（６４．７％）、桑椹胚发育率（３９．９％）和囊胚发育率（２４．８％），说明在成熟培养液中单独添加Ｄ０ＥＣＳ是可行的；在含１０％Ｄ０ＥＣＳ的成熟培养液中再添加１０％或２０％ＢＦＦ（牛卵泡液），均能提高受精卵的卵裂率以及桑椹胚和囊胚的发育率，以添加２０％ＢＦＦ效果较好，其囊胚发育率（３５．０％）显著高于对照组（２４．８％，Ｐ＜０．０５）；在卵泡卵母细胞成熟培养系统和受精卵共同培养系统中添加１０μｇ／Ｌ重组牛生长因子（ｒｂＧＨ），虽对体外受精卵的卵裂率无显著影响（Ｐ＞０．０５），但能显著提高卵裂胚的囊胚发育率（Ｐ＜０．０５）

In this paper, cattle ovaries collected from Zhengzhou slaughterhouse were used, ability of bovine 2￣5 mm follicular oocytes for in vitro maturation (IVM), in vitro fertilization (IVF), in vitro culture (IVC) of fertilized eggs were studied. Single addition of 10% D 0ECS (V/V) in maturation medium obtained higher cleavage rate (64.7%) and development rate of morula and blastocyst (BL) (39.9%, 24.8%, respectively). The results indicated that single addition of 10% D 0ECS is practicable. Addition of 10% D 0ECS and 10% BFF or 20% BFF (V/V) in maturation medium increased the cleavage rate and percentage of morula and BL. Addition of 20% BFF in maturation medium obtained higher BL development rate (35.0%) compared with that of control group (24.8%, P< 0.05). Addition of 10 μg/L rbGH in maturation medium of oocytes and coculture system of fertilized eggs had no significant effect on cleavage rate of fertilized eggs (P>0.05), but increased the BL IVD rate significantly (P<0.05).