摘要
从正常人血中分离单核细胞,分别加入25mg·L-1脂蛋白(LDL,VLDL)及经Cu2+轻度氧化修饰脂蛋白(MM-LDL,MM-VLDL)进行培养.应用免疫细胞化学技术观察蝙蝠葛碱对脂蛋白诱导单核细胞产生血小板源生长因子B(PDGF-B)蛋白的影响.结果表明,LDL和VLDL对单核细胞PDGF-B量无明显影响,MM-LDL,MM-VLDL明显增强单核细胞PDGF-B量,而蝙蝠葛碱对此有显著抑制作用.结果提示,蝙蝠葛碱抗动脉粥样硬化作用可能与抑制SPDGF的作用有关.
Human monocytes were prepared and cultured with native LDL, VLDL and minimally modified LDL (MM-LDL), minimally modified VLDL (MM-VLDL) by 10 μmol·L -1 Cu 2+ . The effect of dauricine on the production of platelet- derived growth factor B (PDGF-B) protein induced by lipoprotein was observed in vitro immunocyto- chemically. The results indicated that native LDL and VLDL had no effect on the production of PDGF-B protein, but MM-LDL and MM-VLDL apparently stimulated the production of PDGF-B protein in monocytes, and the latter effect was markedly inhibited by dauricine. It suggests that the antiathero- sclerotic effect of dauricine may be implicated in its inhibitory effect on PDGF production.
出处
《中国药理学与毒理学杂志》
CSCD
北大核心
1996年第2期123-126,共4页
Chinese Journal of Pharmacology and Toxicology
关键词
蝙蝠葛碱
脂蛋白
生长因子
动脉粥样硬化
dauricine
monocytes
lipoproteins
oxidated
growth factor
platelet-#O&derived
immunochemistry
atherosclerosis