UV－DNA和抗UV－DNA血清的研制及初步应用

The Preperation of UV-DNA and Anti UV-DNA in Sera as well as Their Application

本文用短波紫外线（ＵＶＣ）照射人胎盘和小牛胸腺ＤＮＡ取得ＵＶ－ＤＮＡ（紫外线导致变性的ＤＮＡ），并分别对新西兰家兔进行长达９周的免疫。结果表明：人胎盘ＵＶ－ＤＮＡ未能引起免疫应答，其抗ＵＶ－ＤＮＡ未能检出。而小牛胸腺的抗ＵＶ－ＤＮＡ的效价为１：８。用兔抗小牛胸腺ＵＶ－ＤＮＡ检测３２例红斑狼疮（ＳＬＥ）病人和２１例正常人的血清，发现ＳＬＥ病人血清中ＵＶ－ＤＮＡ的阳性率为６５．６％，正常人为５％。两者的差异有非常显著性意义（Ｐ＜０．０１）。提示血清中ＵＶ－ＤＮＡ可望成为ＳＬＥ诊断和疗效观察及人群接触紫外线时健康监测的特异和敏感指标之一。

The Deoxyribonucleic Acide(DNA)from human placenta and calf thymus were irradiated by ultraviolet C,and became as UV-DNA.And then, New Zealand rabbits were immunized with UV-DNA for nine weeks.The results indicated that the titer of anti UV-DNA injected with calf thymus was1:8,but no anti UV DNA was found in those injected with human placenta UV-DNA. Furthermore,the sera from 32 pateints with systemic lupus erythematosus(SLE)and 20 normal persons were tested with ELISA method.The positive rate of UV-DNA was 65.6% in SLE pateints,and only 5 % in the normalone.This result suggested that UV- DNA in sera may become as one of t he specific and sensitive indexes for SLE diagnosis and the therapy observation as well as in health examination of the persons over-exposed to ultraviolet