纤维粘连蛋白在口腔粘膜白斑中的表达

Effect of fibronectin in oral leukoplakia

目的:探讨纤维粘连蛋白(fibronectin,FN)在口腔粘膜白斑(oral leukoplakia,OLK)中的表达。方法:体外培养正常和口腔白斑上皮细胞及成纤维细胞;酶联免疫反应(ELISA)法测定各细胞培养液中FN的浓度;采用免疫组织化学法、免疫细胞化学法观察FN在正常口腔粘膜、白斑粘膜和鳞癌(oral squamous cellcarcinoma,OSCC)粘膜组织及细胞中的分布;RT-PCR技术观察正常口腔粘膜和口腔白斑粘膜上皮及成纤维细胞中FN的表达。结果:ELISA法测定结果显示,白斑成纤维细胞培养液中FN浓度比正常口腔粘膜成纤维细胞培养液明显降低(P<0.01),而二者上皮细胞培养液中FN浓度差异无统计学意义(P>0.05);RT-PCR检测显示,FN在白斑成纤维细胞中的表达显著下降;免疫组化结果与正常口腔粘膜相比,口腔白斑上皮和结缔组织中FN的表达均有不同程度的减少,但其减损程度低于口腔鳞癌组织。结论:口腔粘膜白斑组织中FN表达减少,在白斑癌变的发生和发展过程中具有一定的意义。

Objective： To study the expression of fibronectin（FN） in oral leukoplakia（OLK）. Methods： Fibroblast and epithelial cells of normal oral mucosa and OLK were cultured in vitro. The level of FN in medium was detected by using ELISA method. The distribution of FN in tissues of normal oral mucosa, OLK and oral squamous cell carcinoma （OSCC） was observed by means of inmmunohistochemistry. The expression of FN in fibroblast and epithelial cells of normal oral mucosa and OLK was observed by using RT-PCR technique. Results： The level of FN in medium of OLK fibroblast was lower than that of normal oral mucosa fibroblast markedly by ELISA （ P 〈 0. 01 ） ; the level of FN was not markedly different between epithelium of OLK and normal oral mucosa. The result of RT-PCR indicated that the expression of FN was obviously derogated in OLK fibroblast. The result of inmmunohistochemistry showed that the expression of FN in OLK epidermas and connective tissues declined incoordinately compared with normal oral mucosa and the distribution was different in them, but the degree of lesion in OLK was lower than that in OSCC. Conclusion： It is significant that the expression and different distribution of FN in OLK lead to OSCC. This provides a new way for treating OLK and interdicting OLK cancerization.