肿瘤特异性抗原MAGE、BAGE、GAGE在子宫内膜癌组织中表达的研究

Expression of MAGE,GAGE and BAGE genes in endometrial carcinoma

目的:研究MAGE、BAGE、GAGE基因在子宫内膜癌组织及正常子宫内膜组织中的表达,并探讨其肿瘤特异性抗原作为子宫内膜癌免疫治疗特异性靶位的可能性。方法:采用逆转录-聚合酶联反应(RT-PCR)技术,检测35例子宫内膜癌组织和15例正常子宫内膜组织中MAGE-1、MAGE-3、BAGE和GAGE1-2基因的表达情况,以-βactin作内参照。结果:35例子宫内膜癌组织中,MAGE-1、MAGE-3、BAGE、GAGE1-2表达阳性率分别为46%(16/35)、49%(17/35)、20%(7/35)和26%(9/35),有77%的标本至少表达其中一种基因。15例正常子宫内膜组织中MAGE-1、MAGE-3、BAGE、GAGE1-2基因表达均为阴性。结论:肿瘤特异性抗原MAGE、BAGE、GAGE基因在子宫内膜癌中有较高的表达,MAGE-1、MAGE-3、BAGE基因的表达与子宫内膜癌的病理分期及分级无相关性,GAGE1-2与子宫内膜癌的分期无关,与分级有关。其可作为子宫内膜癌患者免疫治疗的特异性靶点。

Objective： To investigate the expression of MAGE and BAGE, GAGE genes in tissues of endometrial carcinoma and normal endometrium, and evaluate the possibility of using these genes as molecular markers and as targets of specific immunotherapy for endometrial carcinoma. Methods： Samples of 35 endometrial carcinomas and 15 normal endometria were examined with reverse-polymerase chain reaction（RT-PCR） for the expression of MAGE-1, MAGE-3, BAGE and GAGE1-2. Results： In 35 endometrial carcinoma tissues, 46% （ 16/35）, 49% （17/35）, 20% （7/35） and 26%（9/35）of MAGE-1, MAGE-3, BAGE and GAGE1-2 mRNAs, respectively were expressed. At least one gene transcription was detected in 77% of the endometrial carcinoma samples. While no expression was observed in 15 normal endometrial tissues. There was no significant correlation between the expression of MAGE-1, MAGE-3, BAGE and the clinical stage or histological grade of endometrial carcinoma. There was no significant correlation between GAGE1-2 and the clinical stage too, but there was a significant correlation between GAGE1-2 and the histological grade. Conclusions： Our study indicates that MAGE-1, MAGE-3, BAGE and GAGE1-2 are relatively high expressed in endometrial carcino- ma. These tumor-specific antigens can be used as molecular markers for diagnosis and possible targets for immunotherapy for endometrial carcinoma.