不同氟浓度对培养血管内皮细胞损伤的研究

Endothelial cell injury induced by different concentration fluoride in vitro

目的:观察体外不同氟浓度对人脐静脉血管内皮细胞的损伤作用。方法:体外培养人脐静脉内皮细胞株HUVEC-304,在培养液中加入不同浓度的氟化钠,共分9组,分别为正常对照组及加氟组(120、240、360、480、6007、208、40、960μmol/L),连续培养24 h后,细胞计数并观察细胞形态,MTT比色法检测细胞活性,并检测培养液中NOS、OD、MDA的含量。结果:①氟的浓度为120、240μmol/L时细胞数目明显增多,从600μmol/L开始,内皮细胞数量明显下降,细胞形态呈损伤改变;②氟的浓度在120、240μmol/L时,MTT测定细胞活性明显高于正常对照组,以240μmol/L浓度时最高;浓度在600以上时细胞活力明显低于正常对照组,差异均有统计学意义(P<0.01);③培养液中NO从240μmol/L时开始降低,和正常对照组差异显著,SOD从240μmol/L时开始下降,差异均有统计学意义(P<0.05),MDA的含量逐渐升高。结论:低浓度的氟对脐静脉血管内皮细胞有促进增生作用;高浓度有损伤作用,且随剂量的增高而增重。

Objective： To observe the lesion of human umbihcal vein endothehal ceils （HUVECs） induced by different doses of fluoride. Methods： HUVECs were cultured with fluoride in various concentration （ 120, 240, 360, 480, 600, 720, 840, 960 μmol/L）, 24 hours later, the morphologic changes were observed, the cell activity was measured by MqT method, and the MDA, NO and SOD were determined. Results： The ceil increased when treated with fluoride of 120 μmol/L to 240 μmol/L, whereas decreased with fluoride of 600 μmol/L to 960 μmol/L, and the cells were injured. The ceil activity of the group of 120 μmol/L and 240 μmol/L significantly enhanced compared with the normal control, but that of the group of over 600 μmol/L markedly lowered compared with the normal control. NO and SOD decreased, but MDA increased in the group of over 240 μmol/L. Conclusions： Fluoride of low concentration can promote the ceil activity, but that of high concentration can injure the ceil, and the higher the concentration is, the heavier the iniury is.