芩丹胶囊降压作用及其机制的实验研究

Antagonizing hypertensive effect and mechanism of Qindan capsule on spontaneous hypertensive rats

目的:观察芩丹胶囊对自发性高血压大鼠(spontaneous hypertensive rat,SHR)的降压作用和对血浆内皮素(ET)、降钙素基因相关肽(CGRP)、血浆和血管组织中血管紧张素II(Ang-II)含量的影响,并探讨可能的降压机制。方法:将40只SHR均分为5组:芩丹胶囊大剂量组、小剂量组、牛黄降压胶囊组、卡托普利组和模型组,8只Wistar-Kyoto(WKY)大鼠作为正常对照组,分别给予相应药物;给药12周后观测各组大鼠血压、血浆ET、CGRP及血浆和肠系膜动脉组织中Ang-II的含量。结果:芩丹胶囊大剂量组血压较模型组血压明显降低(P<0.01),与牛黄降压胶囊组和卡托普利组相比差异无统计学意义(P均>0.05);与模型组相比,芩丹胶囊大剂量能够降低SHR血浆ET含量,升高CGRP含量(P均<0.05),且效果优于牛黄降压胶囊和卡托普利;对血浆Ang-II含量无影响(P>0.05),且能够降低肠系膜血管组织中Ang-II含量(P<0.05)。结论:芩丹胶囊对SHR具有良好的降压作用,其机制可能与对血浆中血管活性肽和局部肾素-血管紧张素系统(RAS)的调节有关。

Objective： To observe the antagonizing hypertensive effect of Qindan capsule on spontaneous hypertensive rats （SHRs） and detect its possible mechanism. Methods： Forty SHRs were divided into 5 groups： high and low dose of Qindan capsule groups, Niuhuang Jiangya capsule group, Captopril group and model group, and 8 Wistar- Kyoto （WKY） rats were acted as normal control. Each group was given relevant medicine. Blood pressure （BP） of each group and the level of endothelin （ET）, calcitonin gene-related peptide （ CGRP）, Ang-II in plasma and Ang-II in tissues of mesenteric artery were detected 14 weeks later. Results. BP was lower in high dose Qindan capsule group than in model group （ P 〈 0.01 ）, which was not significantly different compared with Captopril group （ P 〉 0.05） and Niuhuang Jiangya capsule group （ P 〉 0.05）. Compared with model group, Qindan capsule was superior to Niuhuang Jiangya capsule and Captopril in reducing the concentration of ET in plasma and in raising the plasmic CGRP （ P 〈 0.05, respectively） ; Though it had no marked effect on reducing Ang-II in plasma compared with other groups （P 〉 0.05）, it could decrease the concentration of Ang-II in tissues of mesenteric artery （P 〈 0.01 ）. Conclusion： Qindan capsule has satistied effect of antagonizing hypertension. The mechanism may be associated with the regulation of vasoactive peptide in plasma and regional rennin-angiotensin system.