纳米载银无机抗菌剂对义齿基托树脂细胞毒性的影响

Influence of Nano-Silver Base Inorganic Antimicrobial on Cytotoxicity of Denture Base Resin

目的研究纳米载银无机抗菌剂对义齿基托树脂细胞毒性的影响。方法纳米载银无机抗菌剂FUMAT T200-4，按0％（对照组）、1％、2％、3％、4％、5％、7％、10％（V／V）比例添加在加热固化型义齿基托树脂中并分组，采用MTT法检测各组基托树脂对传代培养的小鼠成纤维细胞L-929的细胞毒性，比较抗菌剂添加比例对基托树脂细胞毒性的影响。结果作用于小鼠成纤维细胞后2、4、7d，各抗菌剂添加比例组基托树脂毒性反应为0级或1级，小鼠成纤维细胞相对增殖率分别为93．4％～105．9％、114．2％～147．1％和131．8％～148．8％。结论添加低浓度纳米载银无机抗菌剂FUMAT T200-4（添加比例≤10％）的基托树脂，无明显细胞毒性，具有较好的生物安全性。

Objective To explore the influence of nano-silver base inorganic antimicrobial on cytotoxicity of nano-antimicrobial denture base resin. Methods The experimental groups of denture base resin were mixed with the nano-silver base inorganic antimicrobial FUMAT T2004, whose incorporating doses were 0% （control） , 1% ,2% , 3% ,4% ,5% ,7% and 10% （V/V）. Cytotoxicity in vitro on rat＇ s fibroblast was assayed with MTT method, and the grades were compared. Results After 2, 4 and 7days, the grades of cytotoxicity were 0 or 1, and the cell relative growth rates of experimental groups were 93.4%- 105. 9% , 114. 2%- 147. 1% and 131.8%- 148. 8%. Conclusion When the incorporation ratios of the nano-silver base inorganic antimicrobial FUMAT T2004 were 10% or fewer,the nano-antimicrobial denture base resin does not have cytotoxicity on small rat＇ s fibroblast and shows good biocompatibility.