不同支架材料构建组织工程化口腔黏膜回植动物的比较

Experiment on Transplanted Tissue-Engineered Oral Mucosa in Mice with Different Scaffolds

目的以不同支架材料聚羟基乙酸（PGA）和脱细胞真皮基质材料（Alloderm）构建组织工程化口腔黏膜后回植裸鼠，观察其存活及生长情况。方法人口腔黏膜成纤维细胞（OFC）接种至PGA支架上培养7d后，将口腔黏膜上皮细胞分别与PGA-OFC和Alloderm复合，液面下培养4～7d后，进行气-液界面培养7d，然后回植裸鼠背部皮下。回植后7、14d分别取材观察。结果以PGA和Alloderm为支架构建的组织工程化口腔黏膜均可在裸鼠体内至少存活2周，并与裸鼠自身组织融合生长；在观察的第2周，以PGA为支架构建的组织工程化口腔黏膜出现血管化，以Alloderm为支架构建的组织工程化口腔黏膜上皮分化较为明显。结论PGA孔隙有利于细胞和血管的生长，但其强度随降解而降低；以Alloderm为支架的构建物显示出较清晰的组织学结构。

Objective To evaluate the livability and growth of tissue-engineered oral mucosa with scaffold of polyglycolic acid（PGA） and human cadaveric dermis（Alloderm） in vivo. Methods Tissue-engineered oral mucosa were reconstructed by seeding human oral fibroblast （OFC） onto PGA cultured for 7 days, and seeding human oral keratinocytes on PGA-OFC and Alloderm, respectively. They were cultured for 4 - 7 days submerged and then for another 7 days at air-liquid interface before transplanting into mice. Mice were sacrificed on day 7 and 14 posttransplantation for histological examination. Results Tissue-engineered oral mucosa with scaffolds of PGA and Alloderm could survive in immunodeficient mice for at least two weeks, and integrate with surrounding tissues of the mice. The morphology of epithelia in the former was not evident but with vascularization two weeks posttransplantation, while the latter showed well-balanced epithelia but without distinct vascularization. Conclusion Lacuna of PGA is good for the growth of cells and vessles, but the tenacity reduces with disintegrating. However, the equivalent using Alloderm as scaffold shows better histological fabric.