HLA-DR和HLA-DQ等位基因与口腔扁平苔藓的相关性

Association of HLA-DR/DQ with Oral Lichen Planus

目的研究上海及江浙地区汉族人群人类白细胞抗原(HLA)-DRB1、HLA-DQB1等位基因与口腔扁平苔藓(OLP)发病的关系。方法采用聚合酶链式反应-序列特异性引物(PCR-SSP)法,检测上海及江浙地区44例OLP患者和150例正常对照者的HLA-DRB1、HLA-DQB1等位基因表现频率,所得数据在OLP与正常对照之间以及不同类型OLP患者之间进行比较分析。结果与正常对照组相比,OLP组HLA-DRB1*09和HLA-DRB1*07基因表现频率均明显增高,分别为56.8%vs31.3%(P<0.01)和27.3%vs13.3%(P<0.05);而HLA-DQB1*06基因表现频率则显著降低(18.8%vs42.5%)(P<0.01)。OLP组内比较显示,HLA-DRB1*04表达频率在具有糜烂表现的OLP患者中显著升高,与白纹组相比具有显著性差异(P<0.05)。结论HLA-DRB1*09、DRB1*07等位基因与上海及江浙地区部分人群的OLP易感性相关;HLA-DQB1*06等位基因可能与OLP的抵抗性相关联;HLA-DRB1*04可能与糜烂型OLP的发生有关。

Objective To investigate the association of human leukocytes antigen（HLA）-DR/DQ with oral lichen planus （OLP） in the area of Yangtze River Delta, Methods HLA-DRB1 and DQB1 genotyping of 44 unrelated OLP patients and 150 normal controls were performed by polymerase chain reaction-sequence specific primers （PCR-SSP） method. The data were compared between the OLP group and normal controls, and between different types of OLP patients. Results The frequency of HLA-DRB1 ＊ 09 and HLA-DRB1 ＊ 07 alleles were significantly higher in OLP group than those in normal controls （56.8% vs 31.3% and 27.3% vs 13.3% , P 〈0.01 and P 〈0.05）. How- ever, the frequency of HLA-DQB1 ＊ 06 was significantly decreased in OLP group than that in normal controls （18.8% vs 42.5% , P 〈0.01 ）. HLA-DRB1 ＊ 04 allele was more frequent in OLP patients with erosive manifestation than those with reticular manifestation （P 〈0.05）. Conclusion HLA-DRB1 ＊ 09 and DRB1 ＊ 07 are associated with the development of OLP in the area of Yangtze River Delta. HLA-DQB1 ＊ 06 may be associated with the resistance to OLP, and HLA-DRB1 ＊ 04 with the erosive manifestation of OLP.