期刊文献+

银杏叶提取物对糖尿病大鼠学习、记忆及海马血红素加氧酶1表达的影响 被引量:5

Effects of extract of Gingko biloba leaves on learning,memory and hippocampus HO-1 expression In diabetic rats
原文传递
导出
摘要 目的探讨银杏叶提取物(EGb)对糖尿病大鼠学习记忆及海马血红素加氧酶1(HO-1)表达的影响。方法大鼠腹腔注射链脲佐菌素建立糖尿病模型,6个月后以Morris水迷宫观察其学习记忆能力,以RT-PCR观察大鼠海马HO-1 mRNA的表达,以免疫组织化学法观察海马HO-1蛋白表达。结果大鼠糖尿病6个月后Morris水迷宫成绩下降,海马的HO-1 mRNA表达和蛋白表达水平升高(分别为1.635±0.326 vs 0.978±0.214,7.2±1.7 vs 1.9±0.5,均P<0.01);糖尿病EGb干预组(100、50 mg/kg)大鼠较糖尿病大鼠Morris水迷宫成绩提高,HO-1 mRNA表达和蛋白表达水平降低(100 mg/kg:0.954±0.144,2.0±0.8;50 mg/kg:0.988±0.154,2.5±0.6,均P<0.01)。结论EGb可抑制海马的HO-1表达,提高糖尿病大鼠的学习记忆能力。 Objective To explore the effects of extract of Gingko biloba leaves (EGb) on learning, memory and hippocampal heme oxygenase-1 ( HO-1 ) expression in diabetic rats. Methods The behaviors of streptozotocin-induced diabetic rats were observed by Morris water maze for learning and memory after 6 months of diabetes. The HO-1 mRNA expression and protein expression in hippocampus of diabetic rats were detected by RT- PCR and immunohistochemistry respectively. Results The escape latency time in Morris water maze of diabetic rats was prolonged markedly. HO-1 mRNA and HO-1 protein expressions in hippocampus of diabetic rats were increased ( 1. 635 ± 0. 326 vs 0. 978 ± 0. 214,7.2 ± 1.7 vs 1.9 ± 0.5, respectively, both P 〈 0.01 ). The escape latency times in EGb (100, 50 mg/kg) treated groups were shortened. HO-1 mRNA and HO-1 protein expressions in hippocampus were decreased at the same time as compared with diabetic group (100 mg/kg:0. 954 ±0.144,2.0±0.8;50mg/kg:0.988±0.154,2.5 ±0.6, all P〈0.01). Conclusion EGbcan significantly inhibit HO-1 expression in hippocampus and improve learning and memory dysfunction in diabetic rats.
出处 《中华内分泌代谢杂志》 CAS CSCD 北大核心 2006年第5期472-475,共4页 Chinese Journal of Endocrinology and Metabolism
基金 广西省科学基金项目(科自0542073)
关键词 糖尿病 实验性 学习 银杏叶提取物 海马 血红素氧化酶(脱环) Diabetes mellitus, experimental Learning Extract of Gingko Biloba Hippocampus Heme oxygenase (decyclizing)
  • 相关文献

参考文献12

  • 1Zhou M,Small SA,Kandel ER,et al.Nitric oxide and carbon monoxide produce activity-dependent long-term synaptic enhancement in hippocampus.Science,1993,260:1946-1950.
  • 2Alkadhi KA,A1-Hijailan RS,Malik K,et al.Retrograde carbon monoxide is required for induction of long-term potentiation in rat superior cervical ganglion.J Neurosci,2001,21:3515-3520.
  • 3王晶,姬志娟,赵志炜,赵咏梅,盛树力.APP17肽对D-半乳糖性脑老化模型小鼠学习、记忆功能和海马神经元NT-3、NGF表达的影响[J].中国病理生理杂志,2000,16(6):545-548. 被引量:33
  • 4赵咏梅,徐纯,张岱,赵志炜,姬志娟,盛树力.小鼠糖尿病脑病神经元退变的机制及淀粉样β蛋白前体17肽的作用[J].中华内分泌代谢杂志,2000,16(4):246-249. 被引量:13
  • 5McCoubrey WK,Maines MD.The structure,organization,and differential expression of the gene coding rat heme oxygenase.Gene,1994,139:155-161.
  • 6Roseth S,Hansen TWR,Fonnum E,et al.Bilirubin inhibits transport of neurotransmitters in synaptic vesicles.Pediatric Res,1998,44:312-316.
  • 7Fazdernik TL,Emerson MR,Cross R,et al.Soman-induced seizures:limbic activity,oxidative stress and neuroprotective proteins.J Appl Toxicol,2001,21:S87-94.
  • 8Alonso JR,Cardellach F,Lopez S,et al.Carbon monoxide specifically inhibits cytochrome c oxidase of human mitochondrial respiratory chain.Pharmacol Toxicol,2003,93:142-146.
  • 9Thom SR,Fisher D,Zhang J,et al.Neuronal nitric oxide synthase and N-methyl-D-aspartate neurons in experimental carbon monoxide poisoning.Toxicol Appl Pharmacol,2004,194:280-295.
  • 10Morgen D,Holcomb L,Saad I,et al.Impaired spatial navigation learning in the transgenic mice over-expressing heme oxygenase-1.Brain Res,1998,808:110-112.

二级参考文献10

共引文献42

同被引文献146

引证文献5

二级引证文献48

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部