摘要
用不同浓度葡萄糖和胰岛素干预人近端肾小管上皮细胞(HK-2),ELISA法测培养液上清中TGF-β_1浓度,RT-PCR检测HK-2细胞中TGF-β_1 mRNA表达水平。结果显示高浓度葡萄糖和胰岛素通过不同途径上调HK-2细胞TGF-β_1的表达。
HK-2 cells were cultured with various concentrations of glucose and insulin for 12, 24, 48, 72 h. Transforming growth factor-β1 (TGF-β1) protein in supernatant was measured by ELISA, while TGF-β1 mINA expression was assessed by RT-PCR. Data showed that high concentration of glucose and insulin up-regulated the expression of TGF-β1 in HK-2 cells through different pathways.
出处
《中华内分泌代谢杂志》
CAS
CSCD
北大核心
2006年第5期489-490,共2页
Chinese Journal of Endocrinology and Metabolism
基金
国家自然科学基金(30470821)
山东省科委博士基金(02BS094)