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青石棉诱导BEAS-2B细胞ERK1/2磷酸化表达的研究

Expression of phosphorylated ERK1/2 induced by erocidolite fibers in BEAS-2B cells
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摘要 目的了解青石棉在致癌过程中引起信号转导蛋白变化的特点。方法使用人呼吸道上皮细胞株(human bronchial epithelial cell line,BEAS-2B)体外培养,以终浓度100μg/ml的青石棉和100 nmol/L表皮生长因子(epidermal growth factor,EGF)分别刺激BEAS-2B细胞30和120min,使用特异性抗磷酸化细胞外调节蛋白激酶(extracellular regulated protein kinase,ERK1/2)、ERK激酶(ERK kinase,MEK1/2)和抗总ERK1/2、MEK1/2抗体进行Western免疫印迹,检测相应的蛋白表达水平。结果青石棉刺激BEAS-2B细胞30min后,可诱导磷酸化ERK1/2的快速高表达,且此高表达可持续至120min,与对照组比较,差异有统计学意义(P<0.05);EGF作为诱导磷酸化ERK1/2的阳性刺激物,在30和120min两时段内均可诱导ERK1/2的激活,与对照组比较,差异有统计学意义(P<0.05);任何时间段刺激BEAS-2B,在对照、青石棉和EGF组间,总ERK1/2表达差异无统计学意义(P>0.05)。青石棉刺激BEAS-2B细胞30、120min后,可诱导磷酸化MEK1/2的快速高表达,与对照组比较,差异有统计学意义(P<0.05)。结论青石棉可快速诱导BEAS细胞产生磷酸化ERK1/2、MEK1/2蛋白的高表达,提示MAPKs参与了青石棉所致疾病的过程。 Objective To explore the characteristic of the signal transduction in BEAS cells induced by the crocidolite fibers. Methods The human respiratory airway epithelial cells BEAS-2B were cultured in vitro. The final 100 μg/ml crocidolite concentration and IOnM of epidermal growth factor were cocuhured with BEAS-2B cells for 30 minutes and 120 minutes. Phosphorylated ERK1/2 and MEK1/2 were detected by Western Blotting using specific antibodies. Results A rapid phophorylation expression of ERK1/2(molecular weight at 44 kD and 42 kD, also called as p44 and p42) was observed by treatment of the BEAS-2B cells with 100μg/ml crocidolite or 100 ng/ml EGF( the proven activator of the ERK signaling pathway) at 30 minutes. This phosphorylation could be still detected by incubation the ceils at 2 hours. However no expression was changed for the total ERK 1/2 expression at 30 minutes or 120 minutes. Treatment of BEAS cells with 100 μg/ml crocidolite fiber or 100 ng/ml EGF led to the rapid increased phosphorylation of MEK1/2 at 30 minutes; similarly, the overexpression of MEK1/2 could last 2 hours. Conclusion: The crocidolite induces the MAPK(ERK1/2 and MEK1/2) phosphorylation within a shorter time. It indicates that the MAPKs signals are involved in the process of crocidolite induced damage.
出处 《中华劳动卫生职业病杂志》 CAS CSCD 北大核心 2006年第10期597-600,共4页 Chinese Journal of Industrial Hygiene and Occupational Diseases
基金 美国国家环境保护局 美国北卡罗来那大学联合资助项目(CR829522) 河南省卫生创新人才基金项目(20040002) 国家自然科学基金项目(30571552)
关键词 青石棉 人呼吸道上皮细胞 ERK1/2 MEK1/2 Crocidolite Human respiratory airway epithelial celis ERK1/2 MEK1/2
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