摘要
在两种环境条件下种植以普通玉米自交系丹232和爆裂玉米自交系N04为亲本构建的259个F2:3家系群体,采用SSR标记构建了包含183个标记的玉米遗传连锁图谱,覆盖玉米基因组1 762.2 cM,标记间平均距离为9.6 cM。利用三倍体胚乳遗传模型和区间作图方法对籽粒淀粉含量进行了QTL定位和遗传效应分析,春、夏播条件下共检测到10个QTL,春播条件下检测到的QTL在夏播均被检测到,分别位于第1、3、4、5、7染色体上,可解释淀粉的表型总变异分别为36.84%和72.65%,单个QTL解释表型变异介于4.74%~11.26%。在检测到的QTL中,有2个QTL的遗传作用方式在春播均表现为超显性,而夏播分别为加性和部分显性;其他2个为加性,1个为部分显性,5个为超显性。3个QTL的增效基因来自丹232,其余QTL的增效基因均来自N04。
Two hundred and fifty-nine F2:3 families, developed from the cross between a dent corn inbred line Dan232 and a popcorn inbred line N04, were evaluated for its starch content in the replicated experiments under two environments. One hundred and eighty three pairs of SSR markers were selected to construct a genetic linkage map, covering 1 762.2 cM (centiMorgan) and on the average distance of 9.63 cM between the markers. Quantative trait loci (QTL) were identified and its effects were analyzed, using the triploid endosperm genetic model and the interval mapping method. Ten QTL, detected on chromosome 1, 3, 4, 5 and 7 respectively, were mapped in spring and in summer. All detected QTL in spring were also identified in summer. The phenotypic variation of a single QTL varied from 4.74% to 11.26%, and the total contributions of all QTL under two environments were 36.84% and 72.65%, respectively. Two QTL of all identified QTL in spring showed over-dominant effects, but additive and partially dominant in summer respectively, the amounts of other QTL showing Additive, partially dominant and over-dominant effects were 2, 1, 5 respectively. The positive alleles of three QTL were contributed by the normal corn parent Dan232, and the other positive alleles were contributed by the popcorn parent N04.
出处
《遗传》
CAS
CSCD
北大核心
2006年第11期1401-1406,共6页
Hereditas(Beijing)
基金
河南省科技攻关项目(编号:0122011700)~~