G蛋白偶联受体54 mRNA在雌性性早熟大鼠下丘脑中的表达及其意义

Expression of Hypothalamic G Protein-Couple Receptor 54 mRNA in Female Rats with Precocious Puberty and Its Significance

目的检测GPR54在雌性性早熟大鼠下丘脑中的表达,探讨其在真性性早熟发生发展过程中的作用。方法正常5日龄雌性大鼠40只。随机分为4组,每组10只。实验1组和2组大鼠皮下一次注射300μg达那唑以诱导真性性早熟;检查阴道涂片、体质量及子宫、卵巢质量,用化学发光法测其血清黄体生成素水平;半定量RT-PCR法检测下丘脑GPR54 mRNA表达水平。结果GPR54 mRNA在青春前期组、性早熟青春早期组及性早熟青春中期组大鼠中的表达呈逐渐上升趋势,各组差异具有显著性(F=467.55 P<0.01)。性早熟青春早期组与正常青春早期组GPR54表达无显著性差异(P>0.05)。结论GPR54在性早熟启动和发展过程中可能起重要作用。

Objective To investigate the expression of hypothalamic G protein- coupled receptor 54（GPR54） mRNA in female rats with precocious puberty and its effect on progress of precocious puberty. Methods Forty normal female rats at 5 days of age were divided into 4 groups randomly, 10 rats per group. Model groups were given a single subcutaneous injection of 300μg danazol to induce true precocious puberty. The vaginal smears were examined and weights of body, uterus and ovary were measured; serum luteinizing hormone concentrations were measured by Chemiluminescence. The expression of GPR54 mRNA was assayed by semi - quantitative RT - PCR. Results GPR54 mRNA levels in hypothalamus increased 1.38 fold from prepubertal stage to early precocious puberty（ P 〈 0.01 ） and 1.94 fold to middle precocious puberty（P 〈 0.01 ） ; there was no significant statistical difference between the expresseion GPR54 mRNA level of normal early puberty and early precocious puberty. Conclusion GPR54 may play an important role in onset and development of precocious puberty.