麝香百合LLGLO1基因的克隆和表达

Cloning and Expression of A MADS Box Gene LLGLO1 from Lilium longiflorum Thunb

用RACE方法克隆的麝香百合花发育的GLOBOSA(GLO)类B功能基因LLGLO1,与其他多种单子叶植物的GLO类基因高度同源,且C区具有典型的PI结构基序。通过RT-PCR检测,百合不同组织中的LLGLO1基因表达模式与郁金香的GLO类基因相似。即主要集中在百合第一、二、三轮花器官中表达,心皮和茎中有微量表达,而且随着心皮的成熟,其在心皮中的表达量逐渐增加,但在百合叶片中则未检测到LLGLO1的表达,因此认为LLGLO1在百合花器官中呈特异性表达。LLGLO1在百合第一轮花器官中的表达支持了vanTunen对ABC模型的修正。

A MADS box gene named LLGLO1 was cloned from Lilium longiflorum Thunb by rapid amplification of cDNA ends （RACE）. LLGLO1 was a B function gene homologous to GLOBOSA （GLO）, which had high protein sequence homology with GLOBOSA family genes of other monocotyledon plants. It also had PI motif in the C box region, which was conserved sequence in GLO family gene. The expression of LLGLO1 in different lily tissues was detected by RT-PCR. It was similar to GLO class gene in Tulip, LLGLO1 mainly expressed in the first, second and third whorls of flower organ. It supported the modified ABC model by van Tunen. It was also detected faintly expressed in carpel and stem. The expression of LLGLO1 increased with the carpel development. There was no expression of LLGLO1 in lily leaf, which lead us to consider the LLGLO1 gene specially expressed in lily flower organ.