氮磷浓度对条斑紫菜细胞培养的影响(英文)

THE EFFECT OF CONCENTRATION OF NITROGEN AND PHOSPHORUS ON CELL CULTURE IN PORPHYRA YEZOENSIS

氮和磷对紫菜的细胞生长和发育有很大的影响，本文讨论了不同氮源、不同氮和磷的浓度和不同细胞密度对条斑紫菜细胞培养的存活率和细胞生长的影响。试验结果显示，对于细胞生长用NO3－N作为氮源优于用NH4－N的。前3天培养的细胞对N／P浓度的变化有一些忍耐性，3天后N／P的浓度对细胞存活率有影响，当N／P浓度为18－20／1．8—2.0mgl-1时细胞存活率最高，第6天时，可达到77－80％，细胞分裂速度随N／P的浓度而增加。细胞密度为1－2×105细胞／ml时，其存活率和生长较好，如果密度越高，则存活率和生长就越低。因此，在进行紫菜体细胞育苗生产时，细胞悬浮培养前三天，可加入N／P浓度为20／2．0mgl-1。细胞密度以1—2×105细胞／ml为宜。

Nitrogen and phosphorus have a great influence on cell growth and of development in PorPhyr. This paper discussed the effects of defferent sources of nitrogen, varied concentration of nitrogen and phosphorus, and different cell densities on cell survival ratio and cell growth when ce1ls isolated from thalli on PorPhyr yezoensis cultured in vitro. The results showed that the isolated ce11s grew better in NO, -N than in NH' -N media- For the first 3 days, the cultured ce1ls had some endurance to vary concentration of N and P. After 3 days, the concentration of N/P would innuence the cell survival ratio. The cel1 survival ratio was the highest when the concentration of N/P was 18- 20/1. 8- 2.0 mg l-1. And the ce11 division speed would increase when the concentration of N/P promoted. The cell density of 1 - 2 X 105 cells/ml was better to cell survival ratio and growth, if cell density was too higher, the survivaI ratio would be low and cell growth would be slow. The experiments had laid the foundation of the cell culture and breeding with large sca1e in Porphyra yezoensis.