不同栽培条件黄连的质量分析与评价

Quality analysis and evaluation of Rhizoma Coptidis under different cultivation conditions

目的建立黄连药材HPLC指纹图谱分析方法及含量测定方法,比较分析不同栽培条件、不同产地和不同炮制方法制备的黄连药材的质量。方法采用HPLC-UV方法建立了黄连药材指纹图谱,应用HPLC-MS联用技术指认了主要色谱峰;并对黄连药材中的小檗碱、巴马汀和药根碱进行含量测定。结果指认了黄连药材指纹图谱中7个主要的色谱峰,含量测定结果表明不同的栽培条件中,遮荫条件、生长年限对黄连中小檗碱、巴马汀和药根碱的含量影响较大,而种植密度的影响不是很明显;现代生态技术栽培的黄连中这3种生物碱的含量均高于对照药材或与之基本相当。不同产地的黄连中这3种生物碱的含量差别较大。应用不同炮制方法制备的黄连中这3种生物碱的含量差别不明显。结论现代生态栽培技术替代传统人工搭棚方式栽培黄连是可行的,同时为科学炮制黄连提供依据。

Aim To develop methods for the fingerprint analysis of Rhizoma Coptidis and the determination of berberine, palmatine and jatrorrhizine in Rhizoma Coptidis, and analyze the contents of these three alkaloids in Rhizoma Coptidis under different cultivation conditions, from different areas and processed with different methods. Methods Two methods （HPLC-UV and HPLC-MS） have been developed and used in fingerprint analysis of Rhizoma Coptidis. An HPLC method was used to determine the contents of three alkaloids. Results With HPLC-MS techniques, seven major chromatographic peaks in the fingerprint analysis of Rhizoma Coptidis were identified by their MS spectra and compared with the reference standards. In different cultivation conditions, shading conditions and growing ages have obvious influence on the contents of three alkaloids in Rhizoma Coptidis, while planting density was not the major factor that influenced the contents of three alkaloids. The contents of three alkaloids of Coptidis samples were almost higher than those of Coptidis reference material. For Coptidis samples from different cultivation area, the contents of these three alkaloids were different greatly. For Coptidis samples processed with different methods, the contents of three alkaloids were not influenced obviously by processing methods. Conclusion The results showed that the ecology cultivation method to replace the traditional shading method was feasible and provided the theoretical foundation for scientifically processing Rhizoma Coptidis.