Preparation of molecularly imprinted microspheres by photo-grafting on supports modified with iniferter

Molecularly imprinted microspheres (MIMs) of D-mandelic acid were synthesized in this study. Chloromethylated polystyrene beads modified with iniferter were used as supports for photo-grafting of a molecularly imprinted polymer (MIP) layer. The polymers were characterized by FT-IR, elemental analysis and thermo-gravimetric analysis to testify the polymer formation and its thermal stability. Scan- ning electron micrographs (SEM) show that the MIP particles are mono-dispersed and well-spherical, with the average diameter of 4.38 μm and size distribution coefficient of 1.02. Furthermore, the MIMs were packed in a stainless steel column and evaluated as stationary phases in high performance liquid chro- matography (HPLC). The prepared MIP exhibited a considerable capability of chiral separation between template and its enantiomer. Accordingly, this kind of MIP is likely to have wide applications in chemical sensing, chromatographic analysis and solid phase extraction.

Molecularly imprinted microspheres （MIMs） of D-mandelic acid were synthesized in this study. Chloremethylated polystyrene beads modified with iniferter were used as supports for photo-grafting of a molecularly imprinted polymer （MIP） layer. The polymers were characterized by FT-IR, elemental analysis and thermo-gravimetric analysis to testify the polymer formation and its thermal stability. Scanning electron micrographs （SEM） show that the MIP particles are mono-dispersed and well-spherical, with the average diameter of 4.38 μm and size distribution coefficient of 1.02. Furthermore, the MIMs were packed in a stainless steel column and evaluated as stationary phases in high performance liquid chromatography （HPLC）. The prepared MIP exhibited a considerable capability of chiral separation between template and its enantiomer. Accordingly, this kind of MIP is likely to have wide applications in chemical sensing, chromatographic analysis and solid phase extraction.