利用基因芯片技术研究薯蓣皂苷抗乳腺癌的作用机制

Study the effect of dioscin to breast cancer by cDNA microarray technique

目的:通过基因芯片技术研究薯蓣皂苷对乳腺癌细胞基因表达的影响,初步探索药物作用的靶基因。方法:提取总RNA,通过反转录方法制备cDNA荧光探针,进行芯片杂交,然后对芯片进行图像扫描,最后用ImaGene软件包对荧光信号进行分析。结果:57个有信号的基因克隆中,用药前28个高表达,用药后仅3个基因克隆高表达,28个表达无明显差别,低表达基因克隆26个。结论:薯蓣皂苷干预后,引起细胞-met、HGF/SF、myb和CDK4基因表达丰度下降,提示薯蓣皂苷抗肿瘤细胞增殖可能与抑制细胞-met、HGF/SF、myb基因和CDK4基因表达相关。

Objective：To study the effect of dioscin to breast cancer cytogene expression by eDNA microarray technique, and to explore target genes of drug action initially. Methods：Extracted total RNA, using the method of reverse transcription to prepare cDNA fluorescent probe. Having the array allomixised, then scanned the array for image and analyses the photosignal with the hnaGene package. Results：There are 57 gene cloning that have photosignal. Among the total, 28 gene cloning high-express before medication and after medication only 3 gene cloning high-express, 28 have no significant difference and 26 low-express. Conclusion ：The intervention of dioscin causes the low-expresslon of HGF/SF, Met proto-oncogene, myb and CDK4 genes. It indicates that dioscin＇s inhibition to neoplastic cell multiplication is possibly correlated with down-regulation of HGF/SF, Met proto-oncogene, myb and CDK4 gene.