Cox-2和Survivin基因在非小细胞肺癌中的表达及相关性的研究

Study of the expression and the relationship of Cox-2 and Survivin on non-small cell lung cancer

目的:探讨Cox-2和Survivin基因在非小细胞肺癌中的表达及相关性。方法:利用S-P免疫组化技术检测84例肺癌组织及49例癌旁正常肺组织的Cox-2和Survivin蛋白表达水平,分析Cox-2和Survivin表达与肺癌分级、分期、分型和转移的关系及两者的相关性。结果:Cox-2基因在非小细胞肺癌阳性率为67·9%(57/84),Survivin的阳性率为59·5%(50/84),两者的表达与患者年龄、吸烟史、肿瘤大小、分化程度及TNM无关(P>0·05);在非小细胞肺癌组织中Cox-2与Survivin基因的表达呈正相关(P<0·05,r=0·914)。结论:Cox-2与Survivin在组织中均有较高的阳性表达,两者表达具有相关性,两者可能存在共同的激活机制,构成抑制非小细胞肺癌细胞凋亡的多种途径。

Objective：To explore the expression and the relationship of Cox-2, Survivin genes on non-small cell lung cancer （NSCLC）. Methods：The expression of Cox-2 protein and Survivin protein in NSCLC tissues and the normal lung tissues adjacent to carcinoma from 84 cases of patients with NSCLC were examined by S-P method. Results：The positive rates of Cox-2 and Survivin in NSCLC were 67.9% , 59. 5% , respectively. The expression rate of Cox-2 was positively correlated with that of Survivin in tissues of NSCLC（ r = 0. 914 , P = 0. 001 ）. There were no significant difference between the positive rates of the two genes according to various ages, smoking histories, genders, size, differentiation and TNM stages. Conclusion：There are high expression of Cox-2 and Survivin genes products in the NSCLC tissue with a positive correlation between them. It is speculated that a common activation mechanism may coexists between the two genes, which constructed multiple pathways contributing to the inhibition of apoptosis of NSCLC.