大鼠自发性高血压致病相关基因的初步筛选

Preliminary screening of genes related with disease on spontaneously hypertensive rat

目的:探讨自发性高血压大鼠(SHR)和正常血压大鼠(WKY)肠系膜动脉和肾脏组织中基因表达的差异。方法:提取成年SHR和WKY的二级肠系膜动脉和肾脏总RNA,利用高通量RNA阵列技术(RNA array)检测SHR和WKY二级肠系膜动脉和肾脏组织基因表达水平不同的基因,再用RT-PCR证实部分基因表达的改变。结果:芯片筛选出38条基因,其中上调基因19条,下调基因19条。经RT-PCR验证胰岛素样生长因子结合蛋白5和原肌凝蛋白6基因上调,锌指蛋白10和硬脂酰辅酶去饱和酶1基因下调。结论:筛选出的差异基因可能是高血压的相关基因,深入研究筛选出的相关基因及其功能,将为进一步探讨高血压病相关的致病基因打下良好的基础。

Objective：To evaluate the difference of gene expression between the second-order mesenteric arteries and kidneys of spontaeously hypetensive rat（SHR） and normal rat （Wistar-Kyoto rats, WKY）. Methods： The total RNA samples were obtained from second-order mesenteric arteries and kidneys of SHR and WKY. Microarray was used to determine the level of mRNA expression of two groups. The results were confirmed by RT-PCR. Result： Thirty-eight positive genes were screened from microarray, including 19 up-regulated genes and 19 down-regulated genes. Upregulated genes （insulin-like growth factor binding protein 5, tropomyosin isoform 6） and downregulated （stearoyl-Coenzyme A desaturase 1, ring finger protein 10） were identified by RT-PCR. Conclusion： These positive genes are the genes related to hypertension. These results are helpful for investigating the genes related to disease in hypertension patient.