利用Langendorff灌流装置建立一种离体快速心房起搏模型

A Novel Acute AF Model Was Created by Rapid Atrial Pacing in Langendorff-perfused Isolated Rabbit Hearts

目的:为排除干预因素对其它脏器的影响,制作一种离体的快速心房起搏模型。方法:新西兰大耳白兔12只,随机分为对照组和起搏组(n=6)。开胸取出心脏后,置于Langendorff灌流装置上用台氏液持续灌流,起搏组在灌流的基础上同时给予500次/分的快速心房刺激。分别以400ms、300ms、200ms为起搏周期(S1)测定各组在灌流0h、1h后的心房有效不应期(AERP)。结果:与灌流前(0h)对比,灌流及起搏1h后,起搏组的AERP400、AERP300、AERP200均明显缩短,而单纯灌流组则差别不大;单纯灌流组的AERP400>AERP300>AERP200,而起搏/灌流组的AERP400、AERP300、AERP200三者间无显著差异。结论:起搏离体Langendorff灌流的心脏能够建立有效的快速心房起搏模型。

Objective： To exclude the pharmacological actions to other organs, a novel isolated cardic rapid atrial pacing model was created. Methods： Twelve rabbits were randomly divided into two groups （n = 6 for each） .The hearts were removed and perfused by the Langendorff technique with Tyrode buffer continuously, and pacing the right atrium was performed for lhour in pacing group at the same time. Before and after pacing, atrial effective refractory period （AERP） was determined in all hearts at drive cycle length （DCL） of 400, 300 and 200ms, respectively. Results： Comparing to baseline, AERPs shortened significantly in the pacing group, but in control group, AERP did not obviously change. In control group, pacing at shorter DCL resulted in shortening of AERP, but the adaptation was lost in pacing group. Conclusion： It is a effective and applied AF model by Rapid atripacing in Langendorff- perfused isolated rabbit hearts.