支气管哮喘大鼠心肌细胞p53表达研究

A Study of p53 Expression in Bronchus Asthma Rat’s Myocardium

目的:探讨支气管哮喘心肌损害的机制及法医病理学实践中支气管哮喘猝死者的检测指标。方法:建立大鼠支气管哮喘动物模型,运用免疫组化染色方法观察不同实验条件下大鼠心肌p53表达变化。结果:正常对照组及实验对照组心肌p53呈阴性或弱阳性表达,随着时间的延长无明显的改变;各实验组第2周时心肌p53蛋白阳性面积和积分光密度比正常对照组及实验对照组明显升高(P<0·01),随着时间的延长,阳性表达面积和积分光密度呈增加的趋势,第8周达每实验组的阳性表达最高峰。使用肾上腺素组比使用氨茶碱组各时间段阳性信号面积及积分光密度都明显增加;使用肾上腺素组和使用氨茶碱组各时间段阳性信号面积及积分光密度比不使用药物组明显增加;联合使用肾上腺素氨茶碱组的阳信信号面积及积分光密度比不使用药物组低。结论:支气管哮喘本身对心肌有一定的损害;肾上腺素可加重心肌损害,肾上腺素氨茶碱联合使用对心肌损害较轻;p53免疫组化染色可以作为支气管哮喘猝死的辅助诊断指标。

Objective： To research the mechanism of injury of myocardium, and to find the index to detect the sudden death of asthmatic patient. Methods after establishing the model of asthmatic animals, using the inmmunochemistry to investigate the p53 expression of rats＇ myocardium. Results the p53 expression is negative or weakly positive in control, and with the time prolonging, the expression of p53 is not increasing, in the test gToup, after tow weeks, the p53 expression is increasing obviously than the control, with the time prolonging, the expression of p53 is moro increasing and to achieve the pinnacle at the eighth week. Conclusion asthma has detrimental to the myocardium; adrenaline may aggravate myocardial damage; adrenaline unite ainophylline may lessen myocardial damage; p53 could be assistant targets to diagnose the sudden death of asthmatic.