维生素E琥珀酸酯抑制人翼状胬肉成纤维细胞生长作用的研究(英文)

Inhibition of human pterygium fibroblasts in vitro by vitamin E succinate

目的: 探讨维生素 E 琥珀酸酯(VES) 对体外培养翼状胬肉成纤维细胞增殖的影响, 寻找辅助治疗翼状胬肉和预防翼状胬肉复发的新方法。方法: 翼状胬肉成纤维细胞原代、传代培养,观察 VES 对细胞生长曲线的影响,四甲基偶氮唑盐(MTT) 法检测不同质量浓度 VES 对细胞增殖的影响。结果: 与对照组相比,VES 使细胞生长曲线下移,用10, 20mg/L VES 处理细胞 2, 4, 7d 后,细胞生长抑制率分别为 33.2%和 46.7%、67.9%和 76.8%、81.7%和 89.3%。MTT 法显示 VES 对细胞增殖抑制作用在一定范围内呈剂量 - 效应依赖性。结论: VES 可有效抑制翼状胬肉成纤维细胞的增殖活性。

AIM： To study the antiproliferation of vitamin E succinate （YES） on pterygium fibroblasts in vitro and to find a potential agent for prevention and treatment of primary and recurrence pterygium. METHODS： Primary culture and subculture of pterygium fibroblasts were established in vitra ,and different concentrations of VES （0, 10 and 20mg/L） were added to subcultured fibroblasts, respectively. Influence of VES on the growth curve of fibroblast was observed at day 2, 4 and 7 after treatment of VES. 3- [4,5-Dimethylthiazolzyl]-2,5-Diphenyl Tetrazolium Bromide （MTT） assay at 490nm was used to evaluate the effect of the cells proliferation. RESULTS： The addition of VIES to culture caused the marked descent of growth curve in comparison with the control group, and the inhibiting rate of 10 and 20mg/L of VES was 33.2% and 46.7%, 67.9%,and 76.8%, 81.7% at day 2,4 and 7,respedtely VES Could Obviously inhibit the fibroblast Proliferation in dose-dependent manner by MTT assay. CONCLUSION： VES can significantly inhibit the proliferation of pterygium fibroblast in vitro.