罗勒水提物对阿霉素引起的大鼠心肌毒性的影响

Protective effect of aqueous extracts of Ocimum basilicum L. against adriamycin-induced cardiotoxicity in rats

目的探讨罗勒水提物(OBL)对阿霉素(ADR)所致大鼠心肌损伤的保护作用。方法选用Wistar大鼠50只,随机分为5组,分别为正常组、ADR模型组和OBL75、150、300mg/kg3个剂量治疗组。腹腔注射ADR(5mg/kg,隔日1次共5次)造成心肌损伤模型,测定心肌超氧化物歧化酶(SOD)活性;谷胱甘肽过氧化物酶(GSH-Px)活性及丙二醛(MDA)含量。电镜检查心肌细胞超微结构的改变。结果腹腔注射ADR后可致大鼠心肌明显损害,心肌SOD及GSH-Px活性下降,MDA含量升高,同时出现心肌组织超维结构的损伤。OBL(75、150、300mg/kg)能增加SOD及GSH-Px活性(P<0·05),降低MDA(P<0·05)含量,减轻心肌超微结构的损伤。结论OBL对ADR所引起的心脏毒性有一定的保护作用,其机制可能与保护心肌SOD、GSH-Px活性及清除自由基,防止脂质过氧化有关。

Objective To investigate protective effect of aqueous extracts of Ocimum basilicum L. （OBL） on adriamycin-induced cardiotoxicity. Methods Fifty Wistar rats were divided into five groups randomly receiving intragastric normal saline （control group and ADR group）, 75, 150, 300 mg/kg OBL （OBL groups） once a day for 11 d. Simultaneously, ADR group and three OBL groups were intraperitoneally administered with 5 mg/kg ADR every other day, totally five times. Two hours later after the last dosage, the rat hearts were surgically removed, then myocardial glutathione peroxidase （GSH-Px） activity, superoxide dismutase （SOD） and malondialdehyde （MDA） content were determined and the myocardial ultrastructure was observed under electron microscope. Results Compared with control, MDA was increased （P 〈 0. 05）, while myocardial GSH- Px and SOD activities were decreased by ADR （P 〈 0. 05 ） , respectively. Pathologic changes of myocardial ultrastructure were also observed in ADR group. In contrast, administration of OBL significantly increased myocardial GSH-Px and SOD activities and decreased MDA content （P 〈 0.05 ） , in addition, caused less ultrastructural changes of heart. Conclusion OBL can protect the heart against ADR cardiotoxicity caused by ADR, which mechanism is associated with antioxidant deficit.