神经生长因子在新生大鼠耳蜗的定位及其意义

Distribution and significance of NGF in cochleae of neonate rats

目的 观察神经生长因子在新生大鼠耳蜗中的定位，为进一步研究其在听觉通路中的作用机制提供依据。方法 出生后5～7d健康SD大鼠20只，制备耳蜗石蜡切片，用兔抗小鼠神经生长因子（nerve growth factor，NGF）多克隆抗体行免疫组织化学染色（S-P法），检测NGF在耳蜗中的表达。结果 新牛SD大鼠膜迷路呈类三角形结构，可清晰看见幼稚柯替器、螺旋神经节细胞、蜗轴中的细胞染色阳性，细胞核及细胞质内均有表达。结论 NGF对出生后5～7d大鼠螺旋神经节细胞和毛细胞的存活、成熟密切相关。

Objective To investigate the distribution of NGF in the cochleae of neonate rats and to explore the mechanism of NGF in the auditory path. Methods Twenty healthy neonate SD rats aged 5 -7 d were selected and their cochlear paraffin sections were labeled with a rabbit anti-NGF polyclonal antibody by SP method. Results The membranous labyrinth of neonate rats appeared similar to triangle and NGF expression was observed in immature corti＇ s organ, in spiral ganglion cells and in nerve fibers throughout the cochlea. The distribution located in both cytoplasm and nucleus. Conclusion The distribution of NGF was closely related to survival and maturation of spiral ganglion cells and hair cells in rats aged 5 -7 d.