CD2AP和α-actinin-4在大鼠阿霉素肾病模型中的表达

Expression of CD2-associated protein and α-actinin-4 in rats with adriamycin nephropathy

目的:观察CD2相关蛋白(CD2AP)和α-actinin-4这两种足细胞相关分子在大鼠阿霉素肾病模型中蛋白表达水平的变化,探讨它们与蛋白尿发生发展的相关性。方法:30只SD大鼠随机分为肾病模型组和正常对照组,建立阿霉素肾病模型,在2、4、6、8周,应用光镜、电镜观察肾组织的病理改变,BCA法检测24h尿蛋白排泄量,半定量WesternBlot的方法检测大鼠肾皮质中CD2AP和α-actinin-4的表达。结果:随着肾病模型组大鼠尿蛋白量逐渐增加,肾组织病理改变逐步加重,肾皮质CD2AP的表达量先明显增加,后逐渐降至对照组水平,2、4、6周分别为对照组的3.0、1.8、1.2倍,而α-actinin-4的表达量无明显变化。结论:阿霉素肾病模型大鼠肾皮质CD2AP和α-actinin-4这两种足细胞相关分子的蛋白表达规律不同,前者与蛋白尿的发生发展有一定程度的相关性,后者则与尿蛋白量的变化无关。

Objective： To investigate the expression of CD2-associated protein （CD2AP） and α-actinin-4 in rats with adriamycin nephropathy, and to explore their possible effects on the development of proteinuria. Methods： Thirty SD rats, weighing from 140 to 160 g, were randomly divided into nephmpathy model group and normal control group. The nephropathy model was established by a single intravenous injection of adriamycin （6 mg/kg）. Normal control group received the same volume of 0.9% sodium chloride solu- tion. At the end of the 2nd, 4th, 6th and 8th week after injection, the rats were sacrificed and their kidneys were acquired. The renal histology was observed by light microscope and electronic microscope. The semiquantitative western blot was used to detect the levels of CD2AP and α-actinin-4. Results： The protein levels of CD2AP in nephropathy model groups at the end of 2nd, 4th and 6th week were 3.0, 1.8 and 1.2 times of those in normal control group respectively. However, no appreciable alterations were observed in the expression level of α-aetinin-4 at any time point. Conclusion： The expression of CD2AP is associated with the development of proteinuria in adriamycin nephropathy rats. But there is no relationship between the expression of α-actinin-4 and the increase of proteinuria.