支气管哮喘大鼠模型的建立与气道反应性的测定

Establishment of bronchial asthma model in SD rat and estimation of airway responsiveness

目的:为了探讨支气管哮喘的发病机制,建立一种由卵蛋白(OVA)致敏并诱发的支气管哮喘大鼠模型。方法:24只SPF级SD大鼠随机分为正常对照组和哮喘模型组,每组12只。予卵蛋白腹腔注射并雾化吸入制作SD大鼠支气管哮喘模型。予乙酰胆碱(Ach)行支气管激发试验;以动物呼吸机测定呼气相气道阻力(Re);以病理图像分析系统测定支气管壁厚度、支气管平滑肌厚度和支气管黏膜和黏膜下炎性细胞数。结果:比较2组大鼠Re以及支气管壁厚度、支气管平滑肌厚度和支气管黏膜和黏膜下炎性细胞数,结果显示造模成功;相关分析显示,在80～160μg/kg浓度梯度的Ach激发后,哮喘模型组Re值与其病理形态学改变正相关(P<0.01)。结论:该方法能制备支气管哮喘大鼠模型;通过80～160!g/kg浓度梯度的Ach激发后,Re的倍增可直接代表造模的成功。

Objective： To establish the bronchial asthma model in sprague dawley（SD） rat. Methods： Twenty-four SD rats（SPF） were randomly divided into normal control group and asthmatic model group, 12 rats per group. The asthmatic model of SD rats were established by immunization with intraperitoneally injected and inhaled ovalbumin （OVA）. The bronchial provocation test by acetylcholine was used in each group. Expiratory airway resistance （Re）was measured by using animal respirator. The thicknesses of WA/ Pi,ASM/Pi, and the numbers of eosinophils and lymphocytes were measured. Results： Through the comparison of Re,the thicknesses of WA/Pi and ASM/Pi, and the numbers of eosinophils of each group, the modeling method was confirmed to be successful. The analysis of correlation indicated that Re of asthmatic model group was positively correlated with the thicknesses of WA/Pi and ASM/ Pi and the numbers of eosinophils and lymphocytes while challenged by acetylcholine （80 μg/kg and 160 μg/kg）. Conclusion： The bronchial asthma model in SD Rat might be established successfully. The success of the modeling method might be evaluated directly by the concentration doubling of Re, while challenged by acetylcholine of 80 μg/kg and 160 μg/kg.