组织因子抑制子基因局部转移对兔颈总动脉内膜增生的影响

Effects of local delivery of tissue factor pathway inhibitor (TFPI) gene on neointimal proliferation of rabbit carotid artery after balloon injury

目的:研究组织因子抑制子(TFPI)基因局部转移对兔颈总动脉损伤后内膜增生的影响。方法:36只新西兰纯种雄性大白兔随机分为腺病毒空载组,包载组织因子抑制子(TFPI)基因腺病毒组和生理盐水组(每组12只)。用球囊导管对实验兔行颈总动脉损伤,术后14天取病变血管,免疫组化染色观察表明TFPI在转移局部表达成功;病变最明显处取标本进行HE染色,光学显微镜下观察血管内膜增生,以计算机图像分析系统分析血管内膜﹑中膜和腔面积的变化,计算内膜增生细胞核抗原增殖指数。结果:TFPI基因局部转移后血管内膜面积明显减少,中膜面积不变,血管腔面积增大。结论:TFPI基因局部转移显著抑制颈总动脉损伤处血管内膜的增生,有可能防治再狭窄。

Objective： To evaluate the influence of local delivery of tissue factor pathway inhibitor gene on intimal hyperplasia after balloon injury. Methods： Thirty-six male New Zealand white rabbits were divided into three groups, saline, adenovirus-defect and TFPI gene treated group. Balloon catheter was used to induce injury to the carotid artery of rabbits and saline, adenovirus-defect and TFPI gene were locally delivered on injury site. Fourteen days after operation, the local injury vessel was removed and harvested for pathological analysis and immunohistochemical staining. Automatic image analysis system was used to detect the changes of intimal, medium, luminal area, and the proliferation index of proliferating cell nuclear antigen （PCNA）. Results： Morphological analysis showed intimal hyperplasia remarkably decrease, along with the proliferation index of PCNA significantly reduction and luminal area augment significantly in TFPI gene treated group compared with saline and adenovirus-defect group. Conclusion： Local TFPI gene transferring may reduce the expression of PCNA,and decrease the intimal hyperplasia and enlarge the luminal area in carotid artery of rabbits.