PON1基因微卫星多态性与2型糖尿病的关联研究

Relationship between microsatellite DNA polymorphism of human PON1 gene and type 2 diabetes mellitus

目的:探讨南京地区汉族人群中对氧磷酶1基因(PON1)多态性与2型糖尿病是否存在相关性。方法:选择PON1基因附近高杂合度微卫星DNA多态标志,应用聚合酶链反应及变性聚丙烯酰胺凝胶电泳技术,探察这一微卫星多态在正常对照组与2型糖尿病组中的基因频率分布。结果:该多态位点存在4种等位基因,GT重复序列分别重复12、15、17、20次,病例与对照分析结果表示,两组间等位基因频率总体分布差异有显著性(χ2=14.10,P<0.01)。两组间基因型频率总体分布差异有显著性(χ2=12.72,P<0.01)。结论:南京地区汉族人群中对氧磷酶1基因多态性可能与2型糖尿病相关。

Objective： To explore the relationship between microsatellite DNA polymorphism of human PON1 gene and type 2 diabetes mellitus in Chinese population of Nanjing. Methods- Among Chinese people of Han nationality in Nanjing, 119 patients with type 2 diabetes mellitus and 116 nondiabetic subjects were collected randomly. Polymorphism of microsatellite marker was detected by polymerase chain reaction, polyaerylaimde gel electrophoresis and silver staining to investigate the frequency distribution of PON1 gene microsatellite （GT） in patients with type 2 diabetes mellitus or nondiabetic subjects. Results ：The GT sequences in four types of alleles were repeated for 12,15,17,20 respectively. The PON1 gene alleles frequencies in patients with type 2 diabetes mellitus were significantly different from that in control subject（χ^2 = 14.10,P 〈 0.01）. Conclusion：polymorphism of microsatellite markers in PON1 gene may be associated with type 2 diabetes in Han people.