松果体接触脑脊液神经元的实验研究

Cerebrospinal Fluid-Contacting Neuron of Pineal Gland

为证实大鼠接触脑脊液神经元内视紫红质的存在及光照对其的影响,将大鼠处死后立即取出松果体,通过免疫组织化学方法用荧光显微镜检测视紫红质的存在;用电生理方法来证实光照对松果体上的视紫红质可产生作用。在松果体接触脑脊液神经元及松果体内部有视紫红质反应阳性细胞存在;光照松果体后,可使松果体的神经元诱发放电频率明显增加。并且,与光照前松果体自发放电相比有显著差异。哺乳动物松果体接触脑脊液神经元内存在有视蛋白;松果体除了经典的途径调节褪黑素的释放外,可能还有其他途径:光照松果体,可诱导松果体放电或放电频率增加,从而影响褪黑素的释放。

In order to confirm the existence of rhodopsin in cerebrospinal fluid-contacting neuron and the effect of illumination on it, pineal gland of rat was immediately taken after it was killed and examined the existence of rhodopsin with fluorescence microscope by immunohistochemieal method and confirmed the effect of light on rhodopsin in the pineal gland by electrophysiological method. The results showed that rhodopsin positive ceils did exist in the cerebrospinal fluid-contacting neuron and pineal gland. And illumination induced notable increase of discharge frequency in the pineal gland, and had remarkable differences as compared with its spontaneous discharge before the illumination. Therefore, opsin exists in cerebrospinal fluid-contacting neuron of pineal gland; except for classical path to regulate the release of melatonin, it may also have the other path such as illumination on pineal gland by inducing the increase of the induced discharge or discharge frequency thus to affect the release of the melatonin.