耐氨固氮菌浸种对黄瓜幼苗在干旱胁迫下的保护作用

Soaking cucumber seedlings with in aridity stress ammonia-resistant strains of N_2-fixing bacteria for protection

本试验采用耐氨固氮菌浸种处理黄瓜种子,研究了耐氨固氮菌对黄瓜幼苗抗干旱胁迫的部分生化机理。结果显示,用2%菌液浸种处理黄瓜种子,干旱胁迫下,随着胁迫时间的延长,清水组幼苗叶片相对含水量及叶绿素含量降低,相对电导度、M DA含量升高(P<0.05),SOD与POD活性增强(P<0.05,P<0.01),而CAT活性则先降低后升高;经耐氨固氮菌浸种处理后,叶片SOD,POD,CAT活性相对增强(P<0.05),叶片相对含水量及叶绿素含量降低、相对电导度及M DA含量升高的幅度显著降低(P<0.05)。结果表明,经耐氨固氮菌浸种处理后,黄瓜幼苗生理得到改善,提高了幼苗的保护酶活性,黄瓜幼苗抗干旱能力增强,清除活性氧自由基及有害物质的能力增强。

Biochemistry mechanism of cucumber seedling in aridity stress was studied after being soaked with ammonia-resistant Strains of N2-fixing bacteria. The result showed relative water content and chlorophyll content were reduced （P〈0. 05, P〈0. 01） along with time of aridity. Relative electrical conduct, content of MDA rose and activity of SOD,POD increased（P〈0.05 ,P〈0.01 ）. But CAT increased from decrease at first in water group. The activity of SOD,POD and CAT increased （P〈0. 05）and the risen extent was lower obviously（P〈0. 05）that the content of relative water and chlorophyll decreased and relative electrical conduct and MDA of bacteria group increased compared with water group in aridity stress. The result indicated that the ability of resisting aridity was increased and the activity of protection enzyme of cucumber seedling was enhanced significantly.