蚕豆SCE检测环境诱变剂的方法学研究

Study on Method of Sister Chromatid Exchange in Vicia faba to Detect Environment Mutagen

通过大量探索性试验，对蚕豆根尖细胞ＳＣＥ的Ｂｒｄｕ－Ｆｅｕｌｇｅｎ法的试验条件，如Ｂｒｄｕ浓度、Ｂｒｄｕ标记时间及其长短，稀酸水解浓度、时间和温度等对分色的影响进行了详尽的研究，获得了最佳的实验条件及程序，克服了ＳＣＥ研究沿用的ＦＰＧ法程序复杂，难以普及的缺点。此外，还用其他遗传毒理学方法与本方法进行了比较研究，探索利用该技术检测环境中诱变因素的可能性。

The experimental conditions of the Brdu-Feulgen method of SCE in Vicia faba root which include the content of Brdu,labelling time of Brdu, the impacts on SCE of the content of hydrochloric acid and time and temperatrue for hydrolysis were studied and discussed in this paper.The best experiment conditions and procedure,which overcome the short-comings of FPG method that is complicated in procedure and,hence,difficult to be popularized,were obtained.In addition,such method was compared with other genotoxicology method in order to probe into the possibility of utilizing such technology to detect environment mutagen.