摘要
为测定人红细胞ＮＡＤＨ－细胞色素ｂ５还原酶（ｂ５Ｒ）含量，建立了以兔抗ｂ５Ｒ抗体为包被抗体，ｂ５Ｒ单抗为显示抗体的双抗体夹心ＥＬＩＳＡ。利用此法，首次测得中国正常成人（ｎ＝３０）红细胞胞浆的ｂ５Ｒ含量为２５．６３±８．５４ｎｇ／ｍｇＨｂ。新生儿、遗传性高铁血红蛋白血症患者及其家族半合子成员的红细胞胞浆ｂ５Ｒ含量均低于正常成人水平，甚至低至测不出。结果证实，ｂ５Ｒ酶蛋白量减少是新生儿和遗传性高铁血红蛋白血症患者红细胞ｂ５Ｒ活性降低的机制之一。
ToestablishaquantitativemethodforthedeterminationofhumanerythrocyteNADH-cytochromeb5reductase(b5R)protein.Double-antibodysandwichELISAusingrabbitanti-b5Rantibodyforplatecoatingandhorseradishperoxidase-labeledmonoclonalantibodyagainstb5Rasthereporter.Cytosolicb5Rproteincontentinerythrocytesofnormaladults(n=30)was25.63±8.54ng/mgHbandwasmuchhigerthanthatinnormalneonates(n=5).b5Rwasundectableintheerythrocytecytosolofahereditarymethemoglobinaemiapatient,whosemotheralsoshoweddecreasedb5Rlevel.Theseresultssuggestthatreducedb5Rproteincontentaccountsatleastpartlyforthereducederythrocyteb5Ractivityinbothnormalneonatesandhereditarymethemoglobinaemiapatients.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
1996年第6期292-295,共4页
Chinese Journal of Hematology
基金
解放军总后勤部和卫生部"八五"基金
