牛磺酸对老龄蛋鸡体内抗氧化作用影响的研究

Effects of taurine on antioxidation in old laying hens

试验选用504只59周龄海兰褐蛋鸡,随机分为7组,每组3个重复,每个重复24羽。预试期1周,正试期又分为60～64周和64～68周两阶段,在各组鸡饲粮中分别添加0、0.005%、0.01%、0.02%、0.04%、0.08%、0.16%的牛磺酸,研究牛磺酸对蛋鸡抗氧化能力的影响,并初步探讨其影响机制。研究结果表明:(1)试验前期0.005%、0.16%水平牛磺酸添加组蛋鸡全血谷胱甘肽过氧化物酶(GSH-Px)活性显著升高(P<0.05),且0.16%组在试验后期全血GSH-Px活性也显著升高(P<0.05);添加牛磺酸64周龄蛋鸡血清超氧化物歧化酶(SOD)活性没有升高,68周龄SOD活性升高,并且0.08%组酶活极显著升高(P<0.01)。(2)添加牛磺酸可以显著降低蛋鸡心、脑脂褐质含量(P<0.05),其中0.005%添加效果最好。另外,日粮中添加牛磺酸降低了试验后期蛋鸡血清丙二醛含量,其中0.005%、0.02%、0.04%、0.08%组差异显著(P<0.05)。总体上以添加0.005%水平牛磺酸提高蛋鸡抗氧化能力的效果最佳。

Five hundred and four Halen layers at fifty-ninth weeks old were divided into 7 groups with one-way random design, each group containing 3 replicates （24 birds per replicate）. After the first week of pre-experiment, the formal experiment was divided into two stages of 60 - 64 weeks and 64 - 68 weeks. Group 1 to 7, fed with basal diets, was supplemented with taurine at levels 0, 0. 005,0. O1, 0. 02, 0. 04, 0. 08, and 0. 16%, respectively. The objective of the experiment was to study the effects of different concentrations of taurine on antioxidation function in the layers and analyze the related mechanism. The results showed that： （1） Taurine contents of 0. 005% and 0. 16% increased the activity of blood GSH-Px of the 64 weeks old layers significantly （ P 〈0. 05 ）, while taurine content of 0. 16% increased the activity of blood GSH-Px of the 68 weeks old layers significantly （P 〈 0. 05 ） ; Taurine could not enhance the activity of serum SOD in the former period but could increase the activity of serum SOD in the later period, and 0. 08% of taurine was the best （ P 〈 0. 01 ）. （2） Taurine could reduce the concentration of heart lipofuscin and brain lipofuscin significantly （P 〈 0. 05） and the 0. 005% level was the best. In addition, the diets supplemented with taurine at 0. 005% , 0. 02% , 0. 04% and 0. 08% decreased the concentration of serum MDA in the later period （ P 〈 0. 05）. In conclusion, the group of 0. 005% taurine was the best for improving the antioxidation in old layers.