椰毒假单胞菌及其酵米面亚种诱变性的研究

Study on the mutagenic strains of Pseudomonas cocovenenans and P.cocovenenans subsp.farinofermentans

选取十二烷基磺酸钠或溴化乙锭(EB)为诱变剂,采用高温连续传代培养法处理椰毒假单胞菌株(NCIB 9450)和部分典型的强产毒椰毒假单胞菌酵米面亚种菌株(Co7、Co5、Co14和HN892y 等),筛选出4株失去产毒能力的菌株:Co14S,Co5S,Co7S 和 P.coS。处理后的菌株一般生物学性状没有改变。

Sodium dodecyl sulfate (SDS) and EB were used as mutagens for strains of Pseudomonas cocovenenans and P.cocovenenans subsp farinofermentans,NCIB 9450,Co7,Co5,Co14,and HN892y.After continu- ous subculturing on 37℃,4 mutants,Co14S,Co5S,Co7S and P.CoS,without toxigenicity were screened. Their general biological characters were not changed.