稳定表达外源性p53基因U_(14)细胞系的建立及鉴定

Establishment and Identification of U_(14) Cell Line with Stable Expression of Exogenous p53 Gene Department of Tumor Immunology Research

目的建立稳定表达外源性p53基因的U14细胞系。方法将提取的含p53质粒DNA用脂质体介导的转染方法导入U14细胞,经含G418选择培养基筛选得到稳定转染的细胞系,用间接免疫细胞化学法鉴定外源性p53基因的表达情况。结果筛选得到的U14细胞系可检测到外源性p53蛋白的表达。结论建立了稳定表达外源性p53基因的U14细胞系,为进一步研究p53基因用于肿瘤免疫治疗奠定基础。

Objective To establish U14 cell line with stably expressing exogenous p53 gene. Methods Plasmid DNA containing wt-p53 cDNA was introduced by lipofectin transfection reagent into U14 cell line in vitro. U14 cell line stably expressing wt-p53 protein was selected in the presence of G418. The expression of p53 was detected by indirect immunocytochemistry. Results Exogenous p53 gene was stably expressed in transfected U14 cell. Conclusion The U14 cell line stably expressing p53 gene is established,and it can be used for the further study of p53 gene-immunotherapy of tumor.