玉米品种SSR标记毛细管电泳荧光检测法与变性PAGE银染检测法的比较研究

Comparison of Two Maize SSR Detection Methods: Capillary Electrophoresis with Fluorescence Detection Method and Denaturing PAGE Silver-staining Detection Method

以192份玉米品种为材料,用7对SSR引物对毛细管电泳荧光检测和常规的变性PAGE银染检测两种SSR标记检测方法进行比较分析。结果表明,两种方法检测的SSR标记片段大小一致。毛细管电泳荧光检测法检测的结果更为精确、灵敏、高效,更适用于高通量材料的检测分析。对少量材料进行检测分析以及SSR标记的筛选时,使用常规的变性PAGE银染检测法更经济适用。

Seven SSR primers and 192 maize hybrid were used to comparatively analyze SSR markers between capillary electrophoresis with fluorescence detection and denaturing PAGE （polyacrylamide gel electrophoresis） silver-staining detection. The results indicated that SSR markers fragment sizes explored by two decetion systems were relatively similar. Capillary electrophoresis with fluorescence detection has the advantages of high accuracy, sensitiveness, cost effectiveness and high-throught. Therefore, the capillary electrophoresis with fluorescence detection system is suggested to be suitable for scaning and analyzing large-scale material. While denaturing PAGE silver-staining detection would be used in examaining a few samples, especially in screening SSR markers.