脾虚小鼠脏器组织学变化及强肌健力口服液的修复作用

Morphologic Changes of Organs in Mice with Spleen Deficiency and Their Repair by Qiangji Jianli Oral Liquid

【目的】观察脾虚证模型小鼠肝、脾、肾、胸腺等脏器组织形态学的变化及强肌健力口服液对其修复作用。【方法】将健康雄性NIH小鼠40只随机分为正常对照组、脾虚模型组、强肌健力口服液组和四君子汤组;除正常组外,其他3组均采用腹腔注射利血平法复制脾虚模型,强肌健力口服液组与四君子汤组均按26 g.kg-1.d-1剂量灌胃给药,其他2组灌服等容积蒸馏水,连续21 d;最后1次给药后12 h,各组动物称体质量,取肝、肾、脾、胸腺进行组织病理学观察。【结果】模型组小鼠体质量下降,肝、肾、脾、胸腺均有不同程度的组织损伤;强肌健力口服液与四君子汤均能使模型小鼠体质量增加,使已遭损伤的各脏器组织得以修复。【结论】脾虚模型小鼠的肝、脾、肾、胸腺均呈现不同程度的损伤,而健脾方药对其具有修复作用,验证了《内经》有关“脾主身之肌肉,”“脾为孤脏,中央土以灌四傍”的理论。

[ Objective] To observe the morphologic changes of organs in mice with spleen deficiency and the effect of Qiangji Jianli Oral Liquid （QJOL）. [ Methods] Forty healthy male NIH mice were randomized into 4 groups： A （normal）, B （model）, C （treated with QJOL） and D （treated with Si Junzi Decoction）. Except group A, the mice in other 3 groups received intraperitoneal injection of reserpine to induce spleen deficiency. Meanwhile, groups C and D received QJOL and Si Junzi Decoction in the dosage of 26 g· kg^-1 · d^-1 by gastric garage respectively, and groups A and B received the same volume of distilled water for 21 days. Twelve hours after last administration, the body weight and pathological changes of liver, kidney, spleen and thymus were examined. [ Results ] In group A, the body weight decreased, and liver, kidney, spleen and thymus gland showed different degrees of damage. QJOL and Si Junzi Decoction could increase the body weight and promote the repair of the organs. [ Conclusion ] Different degrees of damage can be found in liver, kidney, spleen and thymus gland of mice with spleen deficiency, and spleen- strengthening medicinal herbs can promote their repair, indicating that the theory of spleen controlling the muscles, being the solitude organ and localized the center to cultivate the surroundings organs like the earth, which is described in Huangdi＇s Canon of Medicine, has the morphologic evidence.