针刺对神经根炎模型大鼠神经功能、血液流变学和炎症因子的影响

Effect of Acupuncture on Neurofunction,Hemorheology and Inflammatory Factors in Radiculitis Rats

【目的】观察针刺郄穴为主对化学性神经根炎模型大鼠的影响,以探讨针刺治疗腰椎间盘突出症的作用机理。【方法】复制化学性神经根炎模型大鼠24只,随机分为针刺组、模型组各12只,并设空白组对照12只;针刺组予以局部针刺夹脊穴,足少阳经、足太阳经郄穴及委中穴治疗,持续28 d,模型组、空白组不予治疗;观察模型大鼠行为学变化及神经细胞形态学改变,测定炎症滤纸质量,并进行血液流变学分析和炎症因子一氧化氮(NO)、白细胞介素-6(IL-6)含量的检测。【结果】针刺能改善模型大鼠患肢活动,促进痛觉、触觉恢复,改善血液流变学各项指标,减轻炎症反应,改善神经根变性,降低炎症因子水平(均P<0.05)。【结论】针刺可改善血液流变学指标、降低NO和IL-6水平、消除神经根炎症及水肿,这可能是临床上针刺郄穴为主治疗腰椎间盘突出的机理。

[Objective] To observe the effect of acupuncture mainly on cleft points on chemical radiculitis rats and to explore the therapeutic mechanism of acupuncture on lumbar disc prolapse. [ Methods] Twenty-four rats with chemical radiculitis were equally randomized into acupuncture group and model group, and another 12 normal rats were in the blank control group. Acupuncture group received acupuncture on acupoints of Jiaji （EX-B2） and Weizhong （BL40） and cleft points of Foot-Shaoyang Meridian and Foot-Taiyang Meridian for 28 days. After treatment, rat behavioral changes and morphological features of nerve cells were observed, the weight of inflammation filter paper was detected, and hemorheologic parameters, nitric oxide （NO） and interleukin 6 （IL-6） were assayed. [ Results ] Acupuncture improved the motor function of affected limb, recovered the senses of pain and tough, improved hemorheologic parameters, reduced the inflammatory reaction, relieved degeneration of nerve root, and decreased the contents of inflammatory factors （P 〈 0.05）. [ Conclusion] The possible therapeutic mechanism of acupuncture on cleft points for lumbar disc prolapse is related to the improvement of hemorheology, decrease of NO and IL-6 contents, and relief of inflammation and edema of nerve root.