采用超高压技术从茶叶中提取茶多酚

Extraction of Tea-polyphenols from Tea Leaves by Ultrahigh Pressure Technique

采用超高压技术常温提取,通用正交试验法,以茶多酚粗品收率为指标,考察溶剂浓度、压力、保压时间、浸泡时间对茶多酚的影响因素,并与回流提取法比较研究茶多酚的超高压提取工艺。结果表明:优化茶多酚的提取工艺条件为:溶剂为60%乙醇。

The extactrion of tea-polyphenols from tea leaves by using the ultrahigh pressure extraction technology （UPE） was studied. The experiment was designed with orthogonal method, and the yield of tea-polyphenols is used as the testing index. The extract conditions, including of the solvent concentration, pressure, the holding time of pressure and the soaking time before extraction, were investigated. The optimum extraction conditions are as follow, 60% ethanol, 200 MPa holding 3 min., soaking 0.5 hr. The yield of tea-polyphenols is 28.92% under the optimum extraction condition. After separation, the yield of crude product of tea extraction is higher 25.3% than that of heat reflux method and the total of tea-polyphenols is higher 32% than that of heat reflux method. In addition, the extraction liquid of UPE is clear, easily to filter and no turbid after stored a long time. As a new extraction technology, UPE showed the great economic benefit if applied to extract tea-ployphenols from tea leaves in industrial scale.