HPLC法测定甲磺酸酚妥拉明的含量

Determination of Phentolamine Mesylate by HPLC

目的：建立高效液相色谱法测定甲磺酸酚妥拉明的含量。方法：采用MACHEREY—NAGEL氰基色谱柱，以0．05mol／L磷酸二氢钾溶液（取磷酸二氢钾6．8g，加水1000ml溶解后，用10％磷酸溶液调节pH值至3．0）-乙腈（4：1）为流动相，流速为1ml／min，检测波长为278nm，进样量20μl。结果：甲磺酸酚妥拉明的线性范围为20～80μg／ml，r=0．9999（n=7），平均回收率为99．49％，RSD=0．38％。结论：本方法快速、简便、重现性好、专属性强，可用于控制甲磺酸酚妥拉明的内在质量。

Objective：To establish an HPLC method for determination of Phentolamine Mesylate. Method：The chromatography was carried out with MACHEREY-NAGEL CN column and mobile phase of 0. 05mol/L potassium phosphate monobasic （weighed 6.8g Potassium phosphate monobasic,added water to 1000ml,adjusted to pH 3.0 with 10% phosphoric acid）-acetonitrile （4 ： 1） with detective wavelength at 278nm. The flow rate of the mobile phase was 1ml/min. Results：The calibration curve was linear in the range of 20-80μg/ml, r = 0. 9999 （n= 7）. The average recovery was 99.49% ,RSD= 0. 38%. Conclusion ：This method is rapid,simple,well repeatable and specific. It can be used as a method of quality control.